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Remove xative, add 2 ml PBSA, and incubate for 5 min. Aspirate PBSA and repeat PBSA wash an additional 2 for 5 min each. Either store at 4 C in PBSA or proceed with staining procedure. Add 1 ml 0.1% Triton X-100 for 10 min at room temperature to permeabilize cells. As previously, wash 3 with 2 ml PBSA for 5 min each. Incubate in 1 ml Blocking Buffer for 15 min at room temperature. Prepare a 1/133 dilution of rabbit anti-rat albumin antibody. For each ml, add 7.5 l antibody to 100 l Blocking Buffer and 892.5 l PBSA. Aspirate Blocking Buffer, add 0.5 ml diluted anti-albumin antibody, and incubate for 2 h at 37 C. Wash 4 with 2 ml Washing Buffer for 5 min each on orbital rocker. Prepare a 1/100 dilution of goat anti-rabbit IgG-FITC. For each ml, add 10 l antibody to 100 l Blocking Buffer and 890 l PBSA. Aspirate nal wash with Washing Buffer, add 0.5 ml of diluted anti-rabbit IgG antibody, and incubate in the dark for 1 h at 37 C. Wash 3 with 2 ml Washing Buffer for 5 min each on rocker, with a nal immersion in PBSA or other standard mounting buffer. Remove coverglass from buffer and mount. Hold coverglass with tweezers, touch edge to towel, and aspirate at edge to remove excess liquid. Turn coverslip upside down and add one small drop Vectashield. Slowly sandwich together coverslip and clean glass slide, and seal coverslip edges with clear nail polish. Store in the dark at 4 C. Prepared specimens should retain uorescent staining for several weeks when stored properly. Examine uorescence, indicating presence of intracellular albumin, using standard uorescence microscopy techniques with FITC lters.

for the chicks of the rst. In this way the female makes the best use of the genetic resources available to her.

Sum of Squares 14,978.521 18.268 14,996.788 14,979.560 17.228 14,996.788

(6:4)

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the source of the stimulus is impaired and it will always orientate towards its intact side irrespective of the position of the prey. Cleary these hairs play a key role in prey capture. Filiform hairs are commonly used as sense organs to detect motion. In the insects each hair is supplied by a single sensory cell that responds to both the strength and the direction of the stimulus. In spiders, however, the arrangement is slightly different with each hair supplied by four cells each of which responds when the hair is de ected in a particular direction. A ying insect generates behind and below it a cone of wind (broader closer to the y and tapering away from it) and so it would seem likely that the trichobothria are involved in some assessment of this air ow (Fig. 2.5). Experiments involving a tethered spider allowed Barth and his team to monitor the pattern of excitation of the sensory cells associated with a single trichobothrium when a y attached to a wire was placed above it. They found that it responded most strongly when it was close to the center of the cone of wind and less strongly if it was moved towards the edge of the cone, or out of it altogether. They also recorded a stronger response to closer ies than to more distant ones, and from winds with higher frequencies and velocities.

Then expand one of the two terminal nodes at random:

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Remote Networks The router at the remote end will also be attached to a DSU/CSU. It will be able to receive the signals generated at the remote end. The typical speeds at which these lines run vary. The most common are 56 Kbps and T1 (1 .544 Mbps) lines, called leased lines because the customer does not own the line. It is leased from the phone company and the rates vary depending on the length of the line. Rates are usually cheaper for short runs (the other point of the network is a few miles away) and more expensive for longer runs. Rates also vary depending on the speed of the line. The serial line provides a simple interconnect between two routers that cannot be connected directly by a LAN. The real problem in using them in an IP internet is that they consume a full network number or a subnet number. There have been methods to overcome this using variable-length subnet masking, which is available with the routing algorithms of OSPF and RIPv2. Otherwise, they generally act as a full network even when there are only two points connected.

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